电化学刺激兔迷走复合区对胆道运动的调节作用

THE EFFECTS OF ELECTRICAL AND CHEMICAL STIMULATION OF DVC ON THE MOTILITY OF BILIARY SYSTEM

为探讨兔迷走复合区（DVC）调节胆道运动的作用，用置入兔胆囊内膀胱法测量胆囊内压（GP）及钩式康铜丝双极电极引导奥迪氏括约肌（SO）肌电。实验结果：（1）电刺激迷走神经背核（DMV）引起GP升高，SO锋电位频率（FSPSO）和SO锋电位幅度（ASPSO）增加；（2）电刺激孤束核（NTS）GP改变不明显，但引起胆囊位相性收缩频率（FRCGB）增加，FSPSO和ASPSO增加；（3）DVC（DMV＋NTS）内微量注射谷氨酸钠（MSG）后GP升高，FPCGB、FSPSO、ASPSO增高，反应持续10～20min后消失；（4）静脉注射阿托品后10min，电或化学刺激DVC不再引起胆囊与SO活动的改变。实验结果提示，DVC对胆道运动有重要的调节作用，该作用可能通过外周M受体介导。

Experiments were performed to investigate the effects of dorsal vagal complex (DVC) on the motility of biliary system in anesthetized rabbits by urethane(1.0g/kg) Mean gallbladder pressure (GP) was measured by a frog bladder filled with normal saline Myoelectric activity of sphincter of Oddi (SO) was recorded with a pair of copper electrodes. The results were as follows: (1)Electrical stimulation of dorsal motor nuclei of the vagus nerve (DMV) significantly increased the GP,the frequency of spike potentials of SO (FSPSO) and the amplitude of spike potentials of SO(ASPSO). (2)Electrical stimulation of nuciel of the solitary tract (NTS) caused no change to the GP,but increased the frequency of phasic contraction of gallbladder (FPCGB),the FSPSO and ASPSO were also enhanced.(3)Microinjection of monosodium glutamate (MSG) into DVC (DMV+NTS) could also raise GP, increase the FPCGB and FSPSO, and enhance ASPSO (4) All the effects mentioned above were abolished by intravenously injectlon of atroplne (0.2mg/kg). These effects indicate that DVC may have a important role in regulating the motility of biliary system via peripheral M receptors.