摘要
Objective The gene expression of skeletal muscle under ischemic condition by direct gene injectionwas observed in order to find a new gene delivery method to treat chronic arterial occlusion disease. Methods Weestablished the rabbit hindlimb ischemic model and used plasmid PSV-β-gal as a reporter gene. We transferedgene intramuscularly and detected the activity of β-galactosidase by histochemistry method. Results Weobserved that the gene expression of skeletal muscle under ischemic condition was higher than normalmuscle. Conclusion The result demonstrated that the direct gene injection was suitable for the chronic peripheralarterial occlusion disease, and might be a novel gene delivery method for this disease.
Objective The gene expression of skeletal muscle under ischemic condition by direct gene injectionwas observed in order to find a new gene delivery method to treat chronic arterial occlusion disease. Methods Weestablished the rabbit hindlimb ischemic model and used plasmid PSV-β-gal as a reporter gene. We transferedgene intramuscularly and detected the activity of β-galactosidase by histochemistry method. Results Weobserved that the gene expression of skeletal muscle under ischemic condition was higher than normalmuscle. Conclusion The result demonstrated that the direct gene injection was suitable for the chronic peripheralarterial occlusion disease, and might be a novel gene delivery method for this disease.
