期刊文献+

CACANA1A突变所致一过性和进展性共济失调对离子通道转运能力及动力学的影响

CACANA1A mutations causing episodic and progressive ataxia alter channel trafficking and kinetics
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摘要 Background: CACNA1A encodes Cav2.1, the pore- forming subunit of P/Q- type voltage- gated calcium channel complexes. Mutations in CACNA1A cause a wide ran ge of neurologic disturbances variably associated with cerebellar degeneration. Functional studies to date focus on electrophysiologic defects that do not adequ ately explain the phenotypic findings. Objective: To investigate whether some mi ssense mutations might interfere with protein folding and trafficking, eventuall y leading to protein aggregation and neuronal injury. Methods: The authors studi ed the functional consequences of two pore missense mutations, C287Y and G293R, in two families with EA2, one newly discovered and the other previously reported . Both mutations caused episodic and interictal ataxia. The biophysical properti es of mutant and wild type calcium channels were examined by whole- cell patch - clamp recordings in transfected COS- 7 cells. The plasma membrane targeting was visualized by confocal fluorescence imaging on Cav2.1 tagged with green fluo rescent protein. Results: The mutant channels exhibited a marked reduction in cu rrent expression and deficiencies in plasma membrane targeting. Conclusions: In addition to altered channel function, the deficiency in protein misfolding and t rafficking associated with the C287Y and G293R mutants may contribute to the slo wly progressive cerebellar ataxia. Background: CACNA1A encodes Cav2.1, the pore- forming subunit of P/Q- type voltage- gated calcium channel complexes. Mutations in CACNA1A cause a wide ran ge of neurologic disturbances variably associated with cerebellar degeneration. Functional studies to date focus on electrophysiologic defects that do not adequ ately explain the phenotypic findings. Objective: To investigate whether some mi ssense mutations might interfere with protein folding and trafficking, eventuall y leading to protein aggregation and neuronal injury. Methods: The authors studi ed the functional consequences of two pore missense mutations, C287Y and G293R, in two families with EA2, one newly discovered and the other previously reported . Both mutations caused episodic and interictal ataxia. The biophysical properti es of mutant and wild type calcium channels were examined by whole- cell patch - clamp recordings in transfected COS- 7 cells. The plasma membrane targeting was visualized by confocal fluorescence imaging on Cav2.1 tagged with green fluo rescent protein. Results: The mutant channels exhibited a marked reduction in cu rrent expression and deficiencies in plasma membrane targeting. Conclusions: In addition to altered channel function, the deficiency in protein misfolding and t rafficking associated with the C287Y and G293R mutants may contribute to the slo wly progressive cerebellar ataxia.
出处 《世界核心医学期刊文摘(神经病学分册)》 2005年第10期53-54,共2页 Digest of the World Core Medical Journals:Clinical Neurology
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