摘要
This study investigated the express ion of vascular en-dothelial growth factor(VEGF)and topoisomerase IIα(TPIIα)in a cohort of serous ovarian carcinomas by im-munohistochemistry with regard to o utcome and clinico-pathologic variables.Formalin -fixed,paraffin -embed-ded archival tissue sections of 10benign serous cystade-nomas and 41serous ovarian carcinom as were immunos-tained with antibodies to VEGF and TP IIα.Immunostain-ing for VEGF was scored with regard to quantity and in-tensity of positively stained cells as weak,focal strong,and diffuse strong.TPIIαlabeling indices(LI )were quanti-tated as the percentage of positively stained nuclei in 1000tumor cells.VEGF expression was wea k or negative in serous cystadenomas.In contrast,f ocal or diffuse strong immunostaining was observed in 21cases(51%)of serous carcinomas.VEGF immunoreac tivity was positively related with TPIIαLI (P =0.0055),adverse outcome (P =0.0052),high International Federation of Gynecol-ogy and Obstetrics(FIGO)stage(P =0.0158),and high tumor grade(P =0.0303).TPIIαLI increased with FIGO stage(P =0.0076)as well as tumor grade(P =0.0034)and indicated poor prognosis(P =0.0182).VEGF immunoreactivity is related wi th TPIIαLI and prognostically relevant.Expression of VEGF protein and TPIIαmay serve as markers of aggressive tu mor behavior in serous adenocarcinomas.
This study investigated the express ion of vascular en-dothelial growth factor(VEGF)and topoisomerase IIα(TPIIα)in a cohort of serous ovarian carcinomas by im-munohistochemistry with regard to o utcome and clinico-pathologic variables.Formalin -fixed,paraffin -embed-ded archival tissue sections of 10benign serous cystade-nomas and 41serous ovarian carcinom as were immunos-tained with antibodies to VEGF and TP IIα.Immunostain-ing for VEGF was scored with regard to quantity and in-tensity of positively stained cells as weak,focal strong,and diffuse strong.TPIIαlabeling indices(LI )were quanti-tated as the percentage of positively stained nuclei in 1000tumor cells.VEGF expression was wea k or negative in serous cystadenomas.In contrast,f ocal or diffuse strong immunostaining was observed in 21cases(51%)of serous carcinomas.VEGF immunoreac tivity was positively related with TPIIαLI (P =0.0055),adverse outcome (P =0.0052),high International Federation of Gynecol-ogy and Obstetrics(FIGO)stage(P =0.0158),and high tumor grade(P =0.0303).TPIIαLI increased with FIGO stage(P =0.0076)as well as tumor grade(P =0.0034)and indicated poor prognosis(P =0.0182).VEGF immunoreactivity is related wi th TPIIαLI and prognostically relevant.Expression of VEGF protein and TPIIαmay serve as markers of aggressive tu mor behavior in serous adenocarcinomas.
