摘要
The purpose of this study was to investigate the expression of epidermal growt h factor receptor and c-erbB-2 oncoprotein in trophoblast populations of place nta accreta. Paraffin sections of 43 placental specimens with (cases) and 43 wit hout (controls) placenta accreta were studied immunohistochemically for epiderma l growth factor receptor and c-erbB-2 expression in the syncytiotrophoblast, v illous cytotrophoblast, and extravillous cytotrophoblast. Epidermal growth facto r receptor and c-erbB-2 protein levels were also performed by Western blot ana lysis. Controls were matched for gestational age in this case-control study. Th e percentage of strong/intermediate immunoreactivity of epidermal growth factor receptor in the syncytiotrophoblast was significantly higher in cases (98%)-th an controls (79%; odds ratio,11.1; 95%CI, 1.3-92.0; P =.03), although strong/ intermediate immunore-activity of c-erbB-2 in the syncytiotrophoblast was sig nificantly lower in cases (35%) than controls (81%; odds ratio, 0.1; 95%CI, 0 .1-0.3; P <.001). Epidermal growth factor receptor and c-erbB-2 expression in the villous and extravillo us cytotrophoblastic cells were not significantly different between controls and cases (P >.05). Most immunoreactive bands on Western blots were consistent with the trends of immunohistochemical findings. The development of placenta accreta is related closely to the differential expression of epidermal growth factor re ceptor and c-erbB-2 in the syncytiotrophoblast.
The purpose of this study was to investigate the expression of epidermal growt h factor receptor and c-erbB-2 oncoprotein in trophoblast populations of place nta accreta. Paraffin sections of 43 placental specimens with (cases) and 43 wit hout (controls) placenta accreta were studied immunohistochemically for epiderma l growth factor receptor and c-erbB-2 expression in the syncytiotrophoblast, v illous cytotrophoblast, and extravillous cytotrophoblast. Epidermal growth facto r receptor and c-erbB-2 protein levels were also performed by Western blot ana lysis. Controls were matched for gestational age in this case-control study. Th e percentage of strong/intermediate immunoreactivity of epidermal growth factor receptor in the syncytiotrophoblast was significantly higher in cases (98%)-th an controls (79%; odds ratio,11.1; 95%CI, 1.3-92.0; P =.03), although strong/ intermediate immunore-activity of c-erbB-2 in the syncytiotrophoblast was sig nificantly lower in cases (35%) than controls (81%; odds ratio, 0.1; 95%CI, 0 .1-0.3; P <.001). Epidermal growth factor receptor and c-erbB-2 expression in the villous and extravillo us cytotrophoblastic cells were not significantly different between controls and cases (P >.05). Most immunoreactive bands on Western blots were consistent with the trends of immunohistochemical findings. The development of placenta accreta is related closely to the differential expression of epidermal growth factor re ceptor and c-erbB-2 in the syncytiotrophoblast.
