摘要
This study was undertaken to investigate whether the increased thrombogenic po tential of cytotrophoblastic cells of women with preeclampsia can be accounted f or by increased rates of apoptosis. Cytotrophoblasts were isolated from the plac enta of (a) nulliparous women without hypertensive disease who were delivered at term and (b) nulliparous women with preeclampsia. The cytotrophoblasts were ide ntified by morphology, and cytokeratin and gonadotropin-releasing hormone posit ivity. The inflammatory cytokine tumor necrosis factor-α.(TNF-α)was added to cytotrophoblasts in vitro and incubated for 24 hours. Tissue factor antigen, activity, and amount of apoptosis were evaluated both before and after TNF-α.stimulation. TNF-α.simulation significantly inc reased tissue factor activity both in the cytotrophoblasts of women with (0.08 ±0.04 pmol/min/106 cells to 0.53 ±.0.19 pmol/min/106 cells) and without (0.07 ±0.04 pmol/min/106 cells to 0.30 ±0.16 pmol/min/106 cells)-preeclampsia. TNF -αstimulation of the cytotrophoblasts also significantly increased tissue fact or antigen in the cytotrophoblasts of both groups of women (3.6 ±0.9 fmol/106 c ells to 34.0 ±7.5 fmol/106 cells, and 7.5 ±1.4 fmol/106 cells to 25.4 ±2.2 fm ol/106 cells, respectively). For both tissue factor antigen and activity, the ma gnitude of increase after stimulation was significantly greater in the preeclamp tic cytotrophoblasts. In contrast, both normal and preeclamptic cytotrophoblasts showed similar increases in their apoptotic indices (approximately 2-fold) aft er induction by TNF-α. The greater response of tissue factor activity and anti gen to TNF-αby preeclamptic cytotrophoblasts cannot be accounted for by the in crease in apoptosis. These data suggest that preeclamptic cytotrophoblasts are i nherently more thrombogenic and more sensitive to TNF-αstimulation.
This study was undertaken to investigate whether the increased thrombogenic po tential of cytotrophoblastic cells of women with preeclampsia can be accounted f or by increased rates of apoptosis. Cytotrophoblasts were isolated from the plac enta of (a) nulliparous women without hypertensive disease who were delivered at term and (b) nulliparous women with preeclampsia. The cytotrophoblasts were ide ntified by morphology, and cytokeratin and gonadotropin-releasing hormone posit ivity. The inflammatory cytokine tumor necrosis factor-α.(TNF-α)was added to cytotrophoblasts in vitro and incubated for 24 hours. Tissue factor antigen, activity, and amount of apoptosis were evaluated both before and after TNF-α.stimulation. TNF-α.simulation significantly inc reased tissue factor activity both in the cytotrophoblasts of women with (0.08 ±0.04 pmol/min/106 cells to 0.53 ±.0.19 pmol/min/106 cells) and without (0.07 ±0.04 pmol/min/106 cells to 0.30 ±0.16 pmol/min/106 cells)-preeclampsia. TNF -αstimulation of the cytotrophoblasts also significantly increased tissue fact or antigen in the cytotrophoblasts of both groups of women (3.6 ±0.9 fmol/106 c ells to 34.0 ±7.5 fmol/106 cells, and 7.5 ±1.4 fmol/106 cells to 25.4 ±2.2 fm ol/106 cells, respectively). For both tissue factor antigen and activity, the ma gnitude of increase after stimulation was significantly greater in the preeclamp tic cytotrophoblasts. In contrast, both normal and preeclamptic cytotrophoblasts showed similar increases in their apoptotic indices (approximately 2-fold) aft er induction by TNF-α. The greater response of tissue factor activity and anti gen to TNF-αby preeclamptic cytotrophoblasts cannot be accounted for by the in crease in apoptosis. These data suggest that preeclamptic cytotrophoblasts are i nherently more thrombogenic and more sensitive to TNF-αstimulation.
