期刊文献+

人受精卵冷冻方案的变化影响胚胎发育和妊娠率

Changes in the freezing protocol for human zygotes alter embryonic development and pregnancy rates
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摘要 To determine the effect of various freezing protocols on postthaw development and pregnancy rates resulting from transfer of human zygotes. Prospective study. Tertiary care center. Couples undergoing assisted reproductive technology (ART) procedures who wished to have their excess zygotes cryopreserved. We cryopreserved zygotes with one of three protocols. Post- thaw survival and development of the zygotes as well as pregnancy rate after transfer of these zygotes. A 3minute hold time after seeding, followed by a final preplunging temperature of - 180℃ , resulted in a clinical pregnancy rate of 28.6% . In contrast, a 15- minute postseed hold time and a - 30℃ final chamber temperature resulted in a 37.3% clinical pregnancy rate. When we combined the protocols to provide a 15- minute postseed holding time and a - 180° C before plunging into liquid nitrogen, we achieved a 69.6% clinical pregnancy rate. By increasing the postseeding hold time and decreasing the temperature of the freezing chamber before plunging the zygotes into liquid nitrogen, significant improvements can be made in postthaw development and pregnancy rates. To determine the effect of various freezing protocols on postthaw development and pregnancy rates resulting from transfer of human zygotes. Prospective study. Tertiary care center. Couples undergoing assisted reproductive technology (ART) procedures who wished to have their excess zygotes cryopreserved. We cryopreserved zygotes with one of three protocols. Post- thaw survival and development of the zygotes as well as pregnancy rate after transfer of these zygotes. A 3minute hold time after seeding, followed by a final preplunging temperature of - 180℃ , resulted in a clinical pregnancy rate of 28.6% . In contrast, a 15- minute postseed hold time and a - 30℃ final chamber temperature resulted in a 37.3% clinical pregnancy rate. When we combined the protocols to provide a 15- minute postseed holding time and a - 180° C before plunging into liquid nitrogen, we achieved a 69.6% clinical pregnancy rate. By increasing the postseeding hold time and decreasing the temperature of the freezing chamber before plunging the zygotes into liquid nitrogen, significant improvements can be made in postthaw development and pregnancy rates.
出处 《世界核心医学期刊文摘(妇产科学分册)》 2005年第7期59-60,共2页 Core Journal in Obstetrics/Gynecology
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