摘要
We examined the practicability, reproducibility and analytical sensitivity of classical immunohistochemistry (IHC) and IHC with microagitation. Two monoclonal antibodies, Ki-67 (proliferation marker) and p53 (tumor suppressor marker), we re used. Consecutive paraffin sections of biopsies of suspicious lesions of pati ents with non-melanoma skin cancer were used in the study. Reproducibility was examined using specimens from four patients in three independent experiments wit h antibodies against Ki-67 and p53. Analytical sensitivity of the two methods w as determined using serial dilutions in two independent experiments. IHC with mi croagitation could be carried out without destroying the tissue. The new techniq ue was consistent and reproducible, and no background staining was observed. The primary antibodies Ki-67 and p53 could be used at higher dilutions (four to te n times) with microagitation compared with classical IHC. Microagitation can be used for immunohistochemistry; it was reproducible, highly sensitive, and antibo dies could be used at higher dilutions. Further analyses with other antibodies u sing this technique are warranted.
We examined the practicability, reproducibility and analytical sensitivity of classical immunohistochemistry (IHC) and IHC with microagitation. Two monoclonal antibodies, Ki-67 (proliferation marker) and p53 (tumor suppressor marker), we re used. Consecutive paraffin sections of biopsies of suspicious lesions of pati ents with non-melanoma skin cancer were used in the study. Reproducibility was examined using specimens from four patients in three independent experiments wit h antibodies against Ki-67 and p53. Analytical sensitivity of the two methods w as determined using serial dilutions in two independent experiments. IHC with mi croagitation could be carried out without destroying the tissue. The new techniq ue was consistent and reproducible, and no background staining was observed. The primary antibodies Ki-67 and p53 could be used at higher dilutions (four to te n times) with microagitation compared with classical IHC. Microagitation can be used for immunohistochemistry; it was reproducible, highly sensitive, and antibo dies could be used at higher dilutions. Further analyses with other antibodies u sing this technique are warranted.
