摘要
Background: Depletion of CD4+CD25+Foxp3+naturally occurring regulatory T cells (Treg) induces autoimmune phenomena. These cells have not yet been fully characterized in the skin of psoriatic patients. Objectives: To prove that the Zenon immunofluorescent labeling technique is suitable for the demonstration of co-localization of T-cell markers and in particular to show the distribution of Treg in psoriatic skin. Methods: In biopsies derived from normal and psoriatic skin, CD4+CD25+, CD4+CD45RO+, CD8+CD25+, CD8+CD45RO+and CD4+CD25+Foxp3+cells in the dermis and in the epidermis were immunophenotyped, using a quantitative immunofluorescent labeling technique (Zenon), analyzed and compared using image analysis. Results: The immuno-fluorescent labeling technique was shown to be an easy and reliable tool to demonstrate co-localization of T-cell markers.In psoriasis, all pathogenic T-cell subsets (CD4+CD25+,CD4+CD45RO+, CD8+CD25+and CD8+CD45RO+cells) were significantly increased in the dermis and in the epidermis, as compared to normal skin (all p < 0.05). Using this labeling technique we were able to reveal CD4+CD25+Foxp3+Treg in psoriatic dermis, but not in the dermis of normal skin (p < 0.0001). Conclusions: The Zenon immunofluorescence technique in combination with image analysis is suitable for the demonstration of co-localization of T-cell markers in tissue. Increased numbers of pathogenic T cells (CD4+CD25+,CD4+CD45RO+, CD8+CD25+and CD8+CD45RO+) were shown in the dermis and epidermis, whereas CD4+CD25+Foxp3+Treg were identified in psoriatic skin with a predilection for the upper dermis.
Background: Depletion of CD4+CD25+Foxp3+naturally occurring regulatory T cells (Treg) induces autoimmune phenomena. These cells have not yet been fully characterized in the skin of psoriatic patients. Objectives: To prove that the Zenon immunofluorescent labeling technique is suitable for the demonstration of co-localization of T-cell markers and in particular to show the distribution of Treg in psoriatic skin. Methods: In biopsies derived from normal and psoriatic skin, CD4+CD25+, CD4+CD45RO+, CD8+CD25+, CD8+CD45RO+and CD4+CD25+Foxp3+cells in the dermis and in the epidermis were immunophenotyped, using a quantitative immunofluorescent labeling technique (Zenon), analyzed and compared using image analysis. Results: The immuno-fluorescent labeling technique was shown to be an easy and reliable tool to demonstrate co-localization of T-cell markers.In psoriasis, all pathogenic T-cell subsets (CD4+CD25+,CD4+CD45RO+, CD8+CD25+and CD8+CD45RO+cells) were significantly increased in the dermis and in the epidermis, as compared to normal skin (all p < 0.05). Using this labeling technique we were able to reveal CD4+CD25+Foxp3+Treg in psoriatic dermis, but not in the dermis of normal skin (p < 0.0001). Conclusions: The Zenon immunofluorescence technique in combination with image analysis is suitable for the demonstration of co-localization of T-cell markers in tissue. Increased numbers of pathogenic T cells (CD4+CD25+,CD4+CD45RO+, CD8+CD25+and CD8+CD45RO+) were shown in the dermis and epidermis, whereas CD4+CD25+Foxp3+Treg were identified in psoriatic skin with a predilection for the upper dermis.
