摘要
对于在生命初期参与免疫反应的免疫信号目前尚未被完全阐明。关于新生儿细胞的特点,人们对有关调节炎症基因和细胞因子产生的转录因子核因子kappa B (NF-κB)的活性目前仍知之甚少。这项研究的目的是明确人脐血单核细胞(CBMC)的NF-κB活化的特点。笔者分析了在早期免疫系统中NF-κB活性与淋巴细胞增生的潜在联系以及对细胞因子分泌的影响。为了确定遗传对新生儿免疫的影响,笔者评价了母亲的过敏反应对NF-κB调节及细胞因子分泌的影响。来自于健康新生儿的CBMC被提纯并用有丝分裂原(n=28)激活, 用电泳方法测定核提取物,用ELISA方法测定细胞因子分泌,用FISH分析排除相关的缘于母亲对CBMC的污染。所有样本均显示了确定的淋巴细胞增生反应,并且经过有丝分裂原刺激后NF-κB活性均有升高和降低。在未受刺激的CBMC中。
The imrnunologic signals participating in immune responses early in life have not been completely elucidated. Re- garding the characterization of neonatal cells, little is known concerning the activity of transcription factor nuclear factor kappa B (NF-κB), which regulates inflammatory genes and cytokine production. The aim of this study was to characterize NF-κB activation in cord blood mononuclear cells (CBMC) . We analyzed the potential association of NF-κB activity with lymphocyte proliferation and influences on cytokine secretion in the early immune system. To determine the contribution of a disease whereby inheritance may impact neonatal immunity, we assessed the influence of maternal allergic disease on NF-κB regulation and cytokine secretion. CBMC from healthy newborns were isolated and stimulated with mitogen (n = 28). Nuclear extracts were analyzed by electrophoretic mobility shift assay, cytokine secretion by ELISA. FISH analysis excluded relevant maternal contamination of CBMC. All samples showed a positive lymphoproliferative response, and NF-KB activity was both increased and decreased after mitogen stimulation. Increased NF-KB activation was significantly associated with decreased TNF-α secretion (median 6. 1 versus 50.3 pg/mL) in unstimulated CBMC. Mitogen stimulation resulted in increased NF-KB activity with a trend to increased IL-13 production. Maternal allergic disease was associated with higher TNF-a (median 982 versus 173 pg/mL) and IL - 13 secretion (median 1328 versus 1120 pg/mL) after mitogen stimulation. Together, NF-KB activity is differentially activated in cord blood and associated with a distinct cytokine pattern. Whether differential NF-KB activity in cord blood is related to the subsequent development of immune diseases requires further investigation.
