摘要
为了明确肠道双歧杆菌数量是否会影响儿童对脊髓灰质炎病毒的免疫反应,笔者进行了一项随机的安慰剂对照试验。从出生到4月龄,给予婴儿服用发酵剂儿童配方(FIF)或标准配方(安慰剂)。每月测量儿童粪便中的双歧杆菌的数目。在接种前及第2次疫苗注射后1 个月,对抗脊髓灰质炎病毒IgA抗体对Pentacoq的反应进行评价。共随机抽取30名儿童,有20名完成了研究(安慰剂组9名,FIF组11名)。FIF组中粪便双歧杆菌水平在4月龄时显著升高(P=0.0498)。而且FIF组婴儿在第4月龄时长双歧杆菌/婴儿双歧杆菌携带量较高(P=0.0399)。经过Pentacoq激发(P【0.001) 后,FIF组的抗脊髓灰质炎病毒IgA滴度显著升高(P【 0.02)。抗体滴度与双歧杆菌数目尤其与长双歧杆菌/ 婴儿双歧杆菌和短双歧杆菌数目(P【0.02)密切相关。体内寄生长双歧杆菌/婴儿双歧杆菌的婴儿同样显示具有较高的抗脊髓灰质炎病毒IgA水平(P【 0.02)。综上所述,目前的结果显示婴儿的抗脊髓灰质炎病毒反应能通过服用发酵配方所激发,该配方有利于肠道双歧杆菌生长。这种对免疫系统的作用是来源于配方中双歧杆菌(主要是长双歧杆菌/婴儿双歧杆菌和短双歧杆菌的激发)的作用还是牛奶发酵所产生的复合物(例如缩氨酸)的作用尚待研究。
To determine whether the size of the intestinal bifidobac-terial population can influence the immune response to poliovirus vaccination in infants, we set up a randomized, placebocontrolled trial. From birth to 4 mo, infants were given a fermented infant formula (FIF) or a standard formula (placebo). Bifidobacteria were quantified monthly in infant stools. Antipoliovirus IgA response to Pentacoq was assessed before and 1 mo after the second vaccine injection. Thirty infants were randomized, and 20 completed the study (nine in the placebo group and 11 in the FIF group). Fecal bifidobacterial level was significantly higher with the FIF group at 4 mo of age (p = 0. 0498) . Furthermore, B. longum/B. inlands carriage was higher at 4 mo in the FIF group (p = 0. 0399) . Antipoliovirus IgA liters increased after Pentacoq?challenge (p < 0. 001), and the rise was significantly higher in the FIF group (p < 0. 02) . Antibody liters correlated with bifidobacteria, especially wilh B. longum/B. infanlis and B. breve levels (p < 0. 002) . Infanls who harbored B. longum/B. in-fantis also exhibited higher levels of antipoliovirus IgAs (p < 0. 002) . In conclusion, the presenl results indicate that anlipoliovirus response can be triggered with a fermented formula that is able to favor intestinal bifidobacteria. Whether this effect on the immune syslem is achieved through the bifidogenic effecl of the formula (mainly through B. longum/B. infantis and B. breve stimulation) or directly linked to compounds (i. e. peplides) produced by milk fermenlalion remains to be invesligaled.
