傅立叶变换红外光谱用于不同状态胆囊组织的初步研究

Preliminary investigation of FT - IR on normal, inflammatory and cancer tissues of gallbladder

目的:探索正常、炎性和癌变胆囊组织各自特异的傅立叶变换红外光谱(FT-IR)表现。方法:应用FT-IR对7例正常胆囊组织、7例炎性胆囊组织和2例胆囊癌组织标本进行检测,总结与组织状态相关的光谱特征。结果:胆囊正常、炎性和癌组织具有不同的FT-IR光谱表现,1550cm^(-1)处的酰Ⅱ带在癌组织中表现较弱,峰形低平,而在正常组织中则较强,峰形高尖。相叶强度Ⅰ1550与Ⅰ1647的比值在7例正常组织中为0.33,0.48.0.54,0.38,0.41,0.52,0.45,炎性组织中为0.32,0.27,0.34,0.25,0.33,0.28,0.29,2例癌组织分别为0.25和0.28;Ⅰ1080与Ⅰ1550的比值在正常组织中为0.059,0.042,0.077,0.085,0.092,0.100,0.081,炎性组织中为0.046,0.040,0.053,0.053,0.048,0.078,0.100,2例癌组织中为0.110和0.170。正常组织中1453cm^(-1)处的峰值高于1402cm^(-1)处,而在癌组织中则相反。结论:胆囊正常、炎性和癌组织的FT-IR表现不同,FT-IR有望成为胆道疾病临床诊断的一种新手段。

Objective: To investigate the special FT - IR spectra of normal, inflammatory and cancer tissue of gallbladder. Methods: Seven normal samples, 7 inflammatory samples and 2 cancer tissues of gallbladder were studied using FT - IR and the special spectra data with different tissues were summarized. Results: Different spectra were found in normal, inflammatory and cancer tissues. The peak at 1550cm-1 was weak and wide in cancer tissue but strong and high in normal tissues. The ratio of 7 1550/I 1647 was 0. 33, 0. 48, 0. 54, 0. 38, 0. 41, 0. 52, 0. 45in 7normal tissues, 0. 32, 0. 27, 0. 34, 0. 25, 0. 33, 0. 28, 0. 29 in 7 inflammatory tissues, 0. 25, 0. 28 in cancer tissues; The ratio of I 1 080/I 1 550 was 0. 059, 0. 042, 0. 077, 0. 085, 0. 092, 0. 100, 0. 081 in 7 normal tissues, 0. 046, 0. 040, 0. 053, 0. 053, 0. 048, 0. 078, 0. 100 in inflammatory tissues and 0. 110, 0. 170 in cancer tissues. The peak at 1453 cm-1 was higher than 1402cm-1 in normal tissues and lower than 1402 cm-1 in cancer tissues. Conclusions: The normal, inflammatory and cancer tissue of gallbladder had different FT - IR spectra. FT - IR can be developed to a new method to diagnose biliary tract diseases.