双重式聚合酶链反应检测印鼠客蚤鼠疫菌的观察

Study on Yersinia pestis in Xenopsylla cheopis Detected by the Double Primers PCR Method

为建立蚤类感染鼠疫的快速诊断方法 ,应用双重式聚合酶链反应 (PL- FI- PCR)技术 ,对感染鼠疫菌的 4 0只印鼠客蚤进行检验 ,同时单体拉胃培养作比较。结果显示细菌培养阳性 2 2只 ,双重式聚合酶链反应阳性 4 0只 ,阳性率后者明显高于前者 ,且特异性扩增带比较清晰、典型、稳定。

This paper reported that the double primers (Pla F1) PCR, bacteria growth were applied in 40 materials of fleas of plague. The results showed that there were 22 of positive in bacteria growth, 40 of positive in the double primers PCR. The PCR positive rate was higher. The bands of PCR products were more typical, clear and stable. It suggested that the double primers (Pla F1) PCR can be applied to detect fleas such smaller insect specimens.