对二氯苯和苯对蚕豆(viciafabaL.)根尖有丝分裂及微核率的效应

The Effect of p-Dichlorobenzene and Benzene on Vicia Faba Root-Pit Cells′ Mitosis and Micronucleus Frequency

作者分别采用5个不同浓度的对二氯苯和苯处理蚕豆根尖，研究其对有丝分裂及微核率的影响，结果表明，各处理浓度均能明显抑制细胞有丝分裂，使有丝分裂指数（MI）显著降低（P＜0.01），同时探讨了MI与微核率（MCNF）的相关性，MCNF随MI的降低而降低，建议在应用蚕豆根尖微核技术（Vicia-MCNT）检测化学物质的毒性时，要充分考虑MI对MCNF的效应，否则可能导致检测结果的偏差，甚至错误的结论。

We treated broadbean(Vicia faba L.)root-pits with different concentrations of p-Dichlorobenzene and Benzene to study the effect of cells’mitosis and the frequency of micronucleus(MCNF).The results indicated that the mitosis index(MI)considerably decreased compared with the controls(CP:lppm,p＜0.01)when the root-tips were treated with the different concentrations of pDichlorobenzene and Benzene.We also discussed the correlations between MI and MCNF nude different conceatrations treated.These suggested that in order to obtain a reliable result in the environmental monitoring and hazarous chemicals detection with icia-MCNT,the affact of MI to MCNF had to be considered,especially,when MI were very low.