摘要
在茶树细胞固体培养研究的基础上，系统分析了摇床转速、接种量、装液量和培养基主要成分对茶树悬浮培养细胞生长及茶氨酸产生的影响。根据试验结果，设计了茶悬浮细胞生产茶氨酸的最佳工艺条件，使茶氨酸含量达到233．25mg／ydry。
Effects of rotation rate,inocula ratio,the bottle load and maior components of the culture medium on the theanine accumulation in the suspension cultured cells were investigated.The operation conditions were optimized. The theanine content in the suspension cells reached as high as 233. 25 mg/gdry.
关键词
茶树
悬浮细胞培养
工艺条件
茶氨酸含量
tea plants
suspension cellculture
technological conditions
theanine content
