银杏内酯对胚基底前脑NOS、AChE阳性神经元发育的影响

EFFECTS OF GINKGOLIDE ON THE DEVELOPMENT OF NOS AND ACHE POSITIVE NEURONS IN EMBRYONIC BASAL FOREBRAIN

目的 探讨银杏内酯对胚基底前脑NOS和AChE阳性神经元发育的影响。方法 实验分成银杏组、NGF组、BDNF组和单纯对照组 ,取孕 17dSD大鼠胚基底前脑原基制成细胞悬液接种于 96孔培养板和 2块 2 4孔培养板中 ,分别加入含银杏内酯、NGF、BDNF及不含上述成份的DMEM培养液 ,于体外培养 18d后 ,96孔培养板行MTT比色分析测定光吸收值 (OD值 ) ,以检测培养的神经元活力。2块 2 4孔培养板分别行NADPH d和AChE组化染色 ,显微镜下计数各组每孔中的NOS和AChE阳性神经元数 ,并用CMM 30 1图像分析系统对两种神经元的细胞面积和细胞周长进行处理 ,数据用方差分析和SNK检验进行统计学处理。结果 银杏组MTT比色分析的OD值和NOS、AChE阳性神经元数、细胞面积、细胞周长等指标均明显地好于单纯对照组 ,达到或仅稍差于NGF组或BDNF组的指标。

Objective To study the effects of Ginkgolide on the development of NOS and AChE positive neurons in the embryonic basal forebrain Method The experimental objects were divided into Ginkgo, NGF, BDNF and control groups The cell suspension of embryonic basal forebrain was prepared from E17 day old SD rats Dissociated cells were placed into a 96 well and two 24 well cultured plates which had DMEM medium containing Ginkgolide, NGF, BDNF or no additive respectively according to the groups. After 18 days of culturing, MTT staining analysis optical density (OD) was undertaken in order to test the vitality of the cultured neurons in the 96 well plate, and the cultured neurons in the two 24 well plates were detected by using NADPH d and AChE histochemical stains respectively The numbers of NOS and AChE positive neurons were counted under microscope, and the cell areas and circumferences of these neurons were analysed with CMM 301 computer image system The data were statistically treated with the analysis of variance (ANOV) and the test of Student Newman Keuls (SNK) Result The OD of MTT staining analysis, and the numbers, the cell areas and circumferences of NOS and AChE positive neurons in the Ginkgo group were strongly superior to those in the control group, and were equivalent to or slightly inferior to those in the NGF or BDNF group Conclusion Ginkgolide was similar effects to those of the neurotrophins such as NGF and BDNF in promoting the development of NOS and AChE positive neurons in the embryonic basal forebrain