中国近缘野生大麦醇溶蛋白的遗传多态性研究

Genetic Polymorphism of Hordein in Wild Relatives of Barley from China

随机选取来源于中国西藏 2 4个不同行政县的 181份近缘野生大麦材料 ,其中包括 4 7份六棱野生大麦、134份二棱野生大麦。选用青藏高原的二棱野生大麦 (W2 )、欧洲的代表品种 Betzes大麦及以色列的二棱野生大麦(Is)作对照。利用 A- PAGE(Acidic- polyacrylam ide gel electrophoresis)法进行了醇溶蛋白遗传多态性的研究。结果表明 ,184份供试材料共得到 6 0种不同的电泳图谱 ,说明西藏近缘野生大麦醇溶蛋白遗传多态性非常丰富。其中有38种图谱为单一材料所独有 ,以 ZYM0 0 19和 ZYM14 88材料为代表的 2种醇溶蛋白图谱占供试材料的 2 9.3% ,在西藏分布较为普遍。聚类分析表明地理环境相似的地区有着相似的图谱类型 ,图谱类型与地理生态环境具有一定的相关性。讨论了中国近缘野生大麦醇溶蛋白的多态性与地理分布的关系 。

Using A PAGE,genetic polymorphism of hordein was analyzed among 181 wild relatives of barley from 24 different counties in Tibet,including 47 six rowed wild relatives of barley and 134 two rowed wild forms.Three barleys that came from Qinghai Province of China,Europe and Israel respectively were used as controls.The hordein patterns showed a very large variation.60 types of hordein patterns were obtained from the tested materials and 38 materials had their unique patterns respectively. The results revealed that 184 materials were clustered into 3 major groups.One major group had only two materials.Two types of hordein patterns that were represented by ZYM0019 and ZYM1488 were widespread and abundant in Tibet,and about 29 3 percent of the tested materials had these patterns.The findings suggested a correlation between hodein patterns and geographic environment.Furthermore,the origin of cultivated barley was discussed.