水稻雄性不育系花药微量热分析

Microcalorimetric Analysis of Anthers of Male Sterile Rice Sterile Line

采用 L KB2 2 77生物活性检测器 ,对核 -质互作雄性不育水稻不育系紫稻 A、野败型不育系珍汕 97A、红莲型不育系红莲粤泰 A和马协型不育系马协 A等 4个不育系以及 1个保持系紫稻 B的花药进行了 37℃条件下等温呼吸曲线的测定。微量热分析结果表明 ,每一不育系都具有一特征曲线 ,等温曲线的幅度高低和走势与花药中花粉粒的败育程度呈正相关 ,这与我们的细胞学观察结果相一致。以紫稻 A败育最彻底 ,其呼吸曲线幅度最低 ,能量代谢最不活跃 ;余者呼吸曲线幅度递增 ,依次为珍汕 97A、马协 A和红莲粤泰 A,最后为紫稻保持系

Using the LKB 2277 bioactivity monitor,five iso thermal curves of anthers including four rice CMS(cytoplasm male sterile) lines Purple rice A,Zhenshan 97 A, Honglian Yuetai A and Maxie A and one maintainer Purple rice B were obtained at 37℃.The results indicated that every curve had its character which was directly correlative to the degree of pollen abortion and consistent with the cytologic analysis, eg.,the numerical value of Purple rice A anther's curve is minimum and the anther's metabolize activity is the weakest correspondingly,and so are the others.This is a new method that can be used in qualitative and quantitative analysis of rice male sterility.