青黛超临界提取物中靛蓝和靛玉红的含量测定研究

Studies on the determination of indigotin and indirubin contents in Indigo Naturalis with Supercritical Fluid Extraction

目的 :本文采用磺化—紫外分光光度法及一阶导数紫外分光光度法 ,对青黛药材及青黛超临界提取物进行含量测定。方法 :样品经磺化反应后 ,用零阶光谱在 6 2 3nm波长处测定靛蓝的含量 ,用一阶导数紫外分光光度法在 4 82nm波长处测定靛玉红的含量。结果 :靛蓝的线性范围为 1 14～ 5 32 μg·ml- 1,r=0 9999,平均回收率为 10 1 0 6 % ,RSD为 1 2 7% ,靛玉红的线性范围为 0 5 2～ 5 2 μg·ml- 1,r =0 9999,平均回收率为 10 1 80 % ,RSD为 0 75 % ,放置 8h对含量测定无影响。结论 :测定数批青黛药材及青黛超临界提取物样品 ,其靛蓝含量测定与药典法测定结果无显著差别。

OBJECTIVE: To determine the contents of indigotin and indirubin in Indigo Naturalis and the extract of Indigo Naturalis with supercritical fluid extraction. METHODS:The indirubin content was determined with sulfonated reaction first order derivative UV spectrophotometry; the indigotin content was determined with sulfonated reaction UV spectrophotometry. RESULTS:The average recovery rate of indirubin was 101 80%, RSD =0 75%, and the average recovery rate of indigotin was 101 06%, RSD =1.27%. CONCLUSTION:This method is simple and repeatable, and can be used as the quality control method for the Indigo Naturalis and the extract of Indigo Naturalis with supercritical fluid extraction.