蛙鸣粉对哮喘豚鼠嗜酸粒细胞凋亡、氧自由基和皮质醇的作用

Effect of Wamingfen on Eosinophil Apoptosis, Oxygen Free Radical and Serum Hydrocortisone Level in Asthma Guinea Pigs

目的探讨蛙鸣粉对哮喘豚鼠嗜酸粒细胞(EOS)凋亡、氧自由基及皮质醇的影响。方法采用卵蛋白致敏诱发豚鼠哮喘,观察中药蛙鸣粉对哮喘豚鼠血浆丙二醛(MDA)、全血谷胱甘肽过氧化物酶(GSH-Px)、红细胞超氧化物歧化酶(SOD)及血清皮质醇(H)含量的影响,以及支气管肺泡灌洗液(BALF)中不同密度嗜酸性粒细胞凋亡百分比。结果蛙鸣粉能提高哮喘豚鼠全血GSH-Px、红细胞SOD、血清H的含量,降低MDA的含量,促进EOS凋亡。结论蛙鸣粉能够有效地提高血清皮质醇的含量,清除氧自由基,促进EOS凋亡,减轻气道炎症引起的气道高反应。

Objective: To explore the effect of Wamingfen on eosinophil apoptosis and oxygen free radicals and to study the influence of hydrocortisone on airway inflammation in asthma guinea pigs. Methods: Guinea pig models of asthma were induced by egg albumin. The effect of Wamingfen on plasma malonyl dialdehyde (MDA), blood glutathione peroxidase (GSH-Px),erythrocytic superoxide dismutase (SOD) activity , serum hydrocortisone level and the percentage of EOS apoptosis in bronchial-alveolar lavage fluid (BALF) were observed. Results: Wamingfen can apparently increase the content of GSH-Px ,SOD and serum hydrocortisone, promote the apoptosis of EOS and reduce the amount of MDA. Conclusion: Wamingfen can effectively increase serum hydrocortisone content, remove the oxygen free radical, promote eosinophil apoptosis and reduce the hyper-reactivity in the inflammatory airway.