豚鼠颞骨三维重建的冰冻连续切片技术

Improved three-dimension reconstruction of serial frozen tissue sections of the temporal bone of guinea pigs

本文介绍了一种改进的豚鼠颞骨冰冻连续切片技术。将颞骨标本用甲酸脱钙,在标本两侧放置定位线,然后将定位线与标本一起用明胶包埋。此技术具有切片薄、制片周期短、定位准确等优点,所得图像既具备切片的细节性,又能反映颞骨的空间特性,对颞骨解剖研究具有重要意义。

Objective To improve the serial frozen tissue sections of the temporal bone of guinea pigs for better three-dimension reconstruction of the computer images. Methods The temporal bone of the guinea pigs was first decalcified by formate before embedding with lines marking the position of the specimen in glutin at low temperature. Result and Conclusion Thinner slices, shorter period and more accurate orientation were achieved to highlight the spacial features of the temporal bone, thus facilitating the anatomical study of the temporal bone.