动脉粥样硬化性脑梗死患者载脂蛋白E基因多态性研究

Reserch on Apolipoprotein E Gene Polymorphism in Patients with Atherosclerotic Cerebral Infarction

目的 :探讨载脂蛋白E(ApoE)基因多态性与动脉粥样硬化性脑梗死的关系。方法 :以聚合酶链反应 -限制性片段长度多态性 (PCR -RFLP)技术结合银染色方法检测了 40例动脉粥样硬化性脑梗死 (ACI)患者和 90例无血缘关系的健康汉族人群载脂蛋白E基因型。结果 :ACI组ε4 等位基因频率明显高于对照组 (P <0 .0 1) ,ε3等位基因频率明显低于对照组 (P <0 .0 1)。结论 :ε4 等位基因是动脉粥样硬化性脑梗死的遗传易患因子之一 ,ε3等位基因是动脉粥样硬化性脑梗死的保护因子。

Objective:To study the relation between Apolipoprotein E(ApoE) polymorphism and atherosclerotic cerebral infarction (ACI). Methods:The ApoE genotypes of 40 ACI patients and 90 cases of unrelated normal Chinese were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) combining silver staining. Results:The frequency of ε 4 allele in ACI was significantly higher than that in the control group(P<0.01), while ε 3 allele was lower(P<0.01). Conclusions:Apoε 4 allele is probably the genetic marker of ACI, while ε 3 allele may be a protective factor.