饮水与肝癌——DNA合成抑制试验检测水质的诱变或致癌作用

DRINKING WATER AND HEPATOCELLULAR CARCINOMA——DNA SYNTHESIS INHIBITION TEST FORDETECTING MUTAGENS AND/OR CARCINOGENS IN WATER

江苏省启东市是我国肝癌高发区之一。自1972年以来,不同作者、不同时间进行多次调查。均发现肝癌发病率高低与饮水类型有关。概括的说,即饮地面水,如宅沟水、泯沟水及河水居民的肝癌发病率显著高于饮井水者。本文应用DNA合成抑制试验(DSI)来检测宅沟水、井水及自来水中的诱变或致癌作用。

Qidong City of Jiangsu Province is one of the endemic areas with highest hepato-cellular carcinoma incidence. A number of authors had studied the relationship betweendifferent sources of drinking water and hepatocellular carcinoma since 1972, and all thedata showed that the risk of drinking ditch water was much higher than that of well water. Potential mutagenesis and/or carcinogenesis related with water were examinedby DNA synthesis inhibition test using V_(79)cell line. N-hydroxyurea and distilled waterwere taken as positive and negative controls.The results showed that the DNA synthesiswas inhibited by well water, tap water, ditch water and N-hydroxyurea with ratesof 32. 65%,46. 85%,62. 89%,and 86. 30% respectively, and had shown a dose-responserelationship. The incidences of hepatocellular carcinoma in the population drinking fromdifferent water sources were 6. 9/10~6 (well water), 20. 8/10~6 (tap water) and 61. 10/10~6(ditch water) respectively, and were coincident with the DNA synthesis inhibitionrates. The result revealed that the above unclean water may contain potential mutagensand/or carcinogens. The principle of DNA synthesis inhibition was discussed.