大肠癌及大肠腺瘤患者染色体不稳定性研究

STUDY ON CHROMOSOME INSTABILITY IN PATIENTS WITH LARGE BOWEL CARCINOMA AND LARGE BOWEL ADENOMA

近年来,随着细胞遗传学和分子遗传学研究的发展,有关染色体不稳定性与肿瘤易感性关系的研究正受到一些学者的兴趣与关注。本文以大肠癌患者、大肠腺瘤患者外周血淋巴细胞染色体为研究材料,观察和比较这些患者的染色体不稳定性特点,井对其与大肠癌易感性的关系进行初步探讨。

The chromosome instability of peripheral lymphocytes was studied in 10 patientswith large bowel carcinoma (LBC), 10 patients with large bowel adenoma (LBA) and 10normal subjects (NS). The chromosome aberration rates (CAR) in different groupswere determined by use of TC 199 medium containing5%fetal bovine serum &0. 098mg/LFudR. The CAR were found to be 6.0±1.49, 3.1±1 .20, 1.1±1.29 in patients withLBC,LBA and NS, respectively,with significant differences among them. The frequen-cies of chromosomal anomalies in patients with LBC were arranged in order as follow:+12,-1P,-18,-5q,-17p;While that in patients with LBA were:-1p,+12,-18,-5q,-17p. The frequencies of sister chromatid exchange (SCE), were 0.198±0. 024, 0.140±0.15 & 0 .103±0.006 in LBC, LBA & NS, respectively. Since both frequencies ofCAR & SCE are higher in LBO & LBA than in NS, it was suggested that the high CARas well as SOE might reflect the genoma instability of LBC & LBA, and chromosomeinstability may be considered as an indicator to detect the subjects susceptible to LBC.