胃癌单克隆抗体MGcl相应抗原的初步研究

STUDY OF THE CORRESPONDING ANTIGEN OF A MONOCLONAL ANTIBODY (MGCI) AGAINST GASTRIC CANCER

本文报告一株鼠抗人胃癌单克隆抗体MGcl相应抗原的纯化及分析。MGcl经50％饱和硫酸铵盐析,DEAE—52纤维素柱层析后,获得纯化的IgG56,31mg与2g溴化氰活化的sepharose 4B偶联,制备免疫吸附剂。用MGcl免疫亲和层析的方法从胃癌组织中纯化出MGcl相应抗原,经蛋白酶处理,高碘酸氧化,电泳脂蛋白袭色,SDS—PAGE及Immunoblotting等实验分析证实MGcl相应抗原是一种低分子量的糖蛋白,分子量为72Kd和52kd。

The corresponding antigen of a monoclonal antibody (MGcl)against gastric cancer was purified and partally characterized. The MGcl was purified by passing throuth a DEAE cellulose column and covalently coupled to CNBr activated Sepharose 4B. By means of immunoaffinity chromatogrphy,the Corr- esponding antigen of MGcl was purified from gastric cancer tissue. The results of periodate oxidation,SDS-PAGEand Immunblotting studies suggest that the corresponding antigen of MGcl is a glycoprotein with molecular weight of 72 KD and 52 KD.