前列腺素E_1和亚硒酸钠对A_(549)肺癌细胞DNA含量的影响

THE EFFECT OF PROSTAGLANDIN E_1 AND SODIUM SELENITE ON THE DNA CONTENT IN A_(549) CELLS IN VITRO

本实验观察了PGE_1和Na_2SeO_3对体外培养的A_(549)细胞DNA含量的影响。实验分为四组:Ⅰ组作为对照组,Ⅱ,Ⅲ,Ⅳ组培养基内分别加入10ngPGE_1/ml,10ngPGE_1和1μgSe/ml,1μgSe/ml。使用显微分光光度计测量结果表明,Ⅱ组(10ngPGE_1/ml)和Ⅳ组(1μgSe/ml)A_(549)细胞内DNA含量明显降低。通过测定单个细胞的DNA含量,我们发现PGE:和Se作用后A_(549)细胞各周期比率发生了改变。Ⅳ组的组方图峰值左移。我们对PFE_1和Na_2SeO_3可能的抑制机制进行了讨论。

The effect of prostaglandin E_1 and Sodium Selenite on the DNA content in A_(549) cells was investigated in vitro. The A_(549) cells were divided into four groups: Groupl was Cultured in the medium alone for control. Group Ⅱ, Ⅲ, Ⅳ were culturet in the medium addedwith 10ngPGE_1/ml, 10ngPGE_1 10ngPGE and 1μgSe/ml, 1μgSe/ml, respectively. By means of UNIVAR microscope photometer, it was found that DNA content in A_(549) cells treated with 10ngPGE_1/ml (group Ⅱ) or lμgSe/ml (groupⅣ) concentration was decreased(P<0.01). By the determination of DNA content in each individual cell, we found that the location of cells in each phase compartment was changed. The peak in group Ⅳ moved to the left. The possible mechanisms of the inhibitory effect ty PGE_1 and Na_2SeO_ were discussed.