鼻咽癌病人Epstein-Barr病毒膜抗原抗体的检测

DETECTION OF ANTIBODIES TO EPSTEI ON BARR VIRUS MEMBRANE ANTI GEN IN SERA FROM PATIENTS WITH NASOPHARYNGEAL CARCINOMA

本文报道,用正丁酸和巴豆油激活人类类淋巴母细胞株—B95—8细胞株、诱导产生早期膜抗原(EMA)和晚期膜抗原(LMA),应用间接免疫荧光法检测鼻咽癌(NPC)病人血清中膜抗原(MA)抗体。MA/IgA在正常人阴性,而在NPC病人阳性率55.96％,GMT 1:20.1。经过葡萄球菌蛋白A(SPA)吸附病人血清后,能提高2～3个血清稀释度。对诊断NPC有意义。

IgG and IgA antibodies to Epstein-Barr virus (EBV) membrane antigen (MA) were detected in sera from 109 nasopharyngeal carcinoma (NPC) patients and normal individuals by indirect immuno-fluorescence test, For MA/IgG antibody, 100％ of NPCpatients were positiva with a GMT of 1: 397.1 and 95.0％ of normal individuals were positive with a GMT of 1: 92.3. In contrast, for MA/IgA antiboby, 55.96％ of NPC patients were positive with a GMT of 1: 20.1 and none of the normal individuals were positive. There was difference in the detectiou of antibodies to EBV MA/IgA when who after preabsorption of sera with staphylococcus aureus (SPA). Our data indicate, that preabsorption of sera with SPA renders the diagnostic test significantly more sensitive for the detection of the NPC and can be used for screening for patients.