中华卵索线虫感染棉铃虫后的血淋巴病理学研究

Study on hemolymph pathology of Heliothis armigera infected by Ovomermis cinensis

棉铃虫 (Heliothisarmigera)被中华卵索线虫 (Ovomermiscinensis)感染之后 ,在第 16小时左右血细胞总数明显增加 ,以浆细胞和粒细胞增多为主 .通过比色法测定在感染后不同时期血淋巴的酯酶活性和可溶性蛋白质含量变化 ,结果表明在感染后的第 4天变化极为显著 ,并采用凝胶电泳技术 ,进一步测定有关病理生化指标的变化 .

The total hemocyte count (THC) of Heliothis armigera infected by Ovomermis cinensis were increased significantly at the sixteenth hour, especially the amount of plasmatocytes and graulocytes. Results show that esterase activities and soluble protein of hemolymph changed greatly on the fourth day by spectrophotometric method. The hemolymph pathological biochemistry changes were further measured by means of gel electrophoresis.