乳腺癌ER和PR测定的ABC法与亲和酶标法对比研究

COMPARATIVE STUDY OF ABC AND AFFINITY ENZYME LABELED METHODS ASSAYING FOR ESTROGEN AND PROGESTIN RECEPTORES IN HUMAN BREAST CANCER

本文运用Avidin-Biotin—Peroxidase Complex(ABC)法和亲和酶标法对50例乳腺癌的冰冻组织和石蜡包埋组织进行雌激素受体(ER)和孕激素受体(PR)测定。结果发现:ABC法与亲和酶标法测定的ER阳性符合率为91.2％。PR为82.8％;ER的阴性符合率为81.3％,PR为(?)6.2％,结果表明二种方法在测定乳腺癌的ER和PR有一致性,运用ABC法测定石蜡包埋的乳腺癌组织中ER和PR具有可靠性和敏感性,这对临床判定预后和内分泌治疗有重要意义。

Fifty cases of the breast cancer were analyzed for Estrogen and Progestin receptors by Avidin-Biotin-Peroxidase Complex (ABC) and affinity enzyme labeled methods on the frozen tissue and paraffin-embdded tissue of the tumor. It is suggested that ER-positive rates of conform of ABC with the affinity enzyme labeled methods were 91.2％, PR were 82.8％; ER-negative rates of the conform were 81.3％, PR were 76.2％. These results suggest that there are the correlation of two methods assaying ER and PR in breast cancer. It is dependable and susceptible to assay ER and PR in paraffinembedded tissue of the breast cancer by ABC method. These results are important in taking clinical endocrine therapy and estimating prognosis of breast cancer.