孕早期母体外周血及胚胎周围辅助T细胞亚群分泌功能

Function of cytokines secreted by helper T cell subsets from maternal peripheral blood and para-embryo during early pregnancy

目的 :分析早孕妇女外周血、宫腔血及蜕膜中的辅助T细胞 (Th)亚群分泌功能。方法 :采用酶联免疫吸附试验 (ELISA)法检测Th1型细胞因子γ干扰素 (IFNγ)及Th2型细胞因子白细胞介素 4 (IL - 4 )的活性。结果 :早孕妇女外周血、宫腔血及蜕膜中淋巴细胞经植物血凝素 (PHA)诱导后 ,三者IFNγ水平较正常未孕妇女明显降低 (P <0 0 1 ) ,后二者较前者明显降低 (P <0 0 1 ) ,而后二者间无显著性差异 (P <0 0 5) ;IL- 4水平 ,前者较正常未孕妇女明显增高 (P <0 0 1 ) ,后二者未检测到有IL - 4。结论 :1 早孕期间Th1型细胞因子IFNγ分泌水平降低 ;而Th2型细胞因子IL - 4分泌水平升高。提示妊娠早期母体可能通过复杂的细胞因子网络 ,抑制Th1型的细胞因子 ,而趋向于Th2型细胞因子的调控 ,从而使妊娠正常进行。 2 早孕期间胚胎周围 (宫腔血和蜕膜 )Th亚群分泌水平与母体外周血间存在差异 ,提示可能与人绒毛膜促性腺激素 (hCG)

Objective:To analyze T helper subsets cytokine secreting function of peripheral blood lymphocytes(PBL) and uterine blood lymphocytes and decidual lymphocytes during early pregnancy.Methods:Enzyme linked Immunosorbent Assay(ELISA) was applied to determine the activity of Th1 type cytokine IFNγ and Th2 type cytokine IL 4.Results:The level of IFNγ in phytohemagglutinin(PHA) induced PBL and uterine blood lymphocytes and decidual lymphocytes from early pregnant women is significantly lower than that of normal unpregnant women( P <0 01).The level of IFNγ in uterine blood and decidua is significantly lower than that of PBL from early pregnant women( P <0 01),but the level of IFNγ in uterine blood and decidua is not different( P >0 05).The level of IL 4 in PBL from early pregnant women is significantly highter than that of PBL from normal unpregnant women( P <0 01).The level of IL 4 in uterine blood and decidua can not be determined.Conclusion:The level of Th1-type cytokine in PBL of early pregnancy is significantly lower,that of Th2-type cytokine is significantly highter.This indicates that maternal immune system during early pregnancy may inhibit Th1 type cytokine and approach Th2 type cytokine regulation to maintain normal pregnancy by complicated cytokine net.The function of cytokines secreted by Th subsets is different between para embryo(uterine blood and decidua)and maternal peripheral blood during early pregnancy.This indicates that it is likely due to the inhibitory effect of hCG.