葛根中不同提取物对外周血免疫细胞R调节机理研究

Studies of Bi-derectional Modulation Effect of Kudzuvine Root on Immunol Cells

目的 :探讨葛根对免疫系统调节作用的机理。方法 :利用鲁米诺介导的化学发光方法 ,研究葛根的不同提取部分 (水溶性部分 ,醇溶性部分 )对PMA刺激人外周血中淋巴细胞 (LC)及嗜酸细胞 (EC)产生活性介质的影响。结果 :水溶性部分对LC及EC具有激活作用 ,可以促进PMA体外刺激LC和EC产生多量活性介质 ,而且其增强作用与水溶部分浓度呈正相关 ;但醇溶部分则相反 ,它对PMA刺激LC ,EC所产生的活性介质具有明显抑制作用 ,这种抑制作用强度同样与醇溶部分浓度相关。结论 :同一种中草药葛根对实验细胞具有双向作用 ,其物质基础来自于它所具有的不同化学成分。

Objective: To evaluate bi directional modulation effect of Chinese herbal medicine on immunol cells. Method: Two different active portions were isolated from Kudzuvine Root(Radix puerariae), one being the ethanol extraction and another the water extraction. Different concentration of these two different portions was studied by using PMA stimulated lymphocyte or eosinophil initiated chemiluminescence system. Result: Water extraction of Kudzuvine Root could enhance chemiluminescence concentration dependently whereas enthanol extraction of Kudzuvine Root inhibited the chemiluminescence significantly. Conclusion:The bi directional regulation effect of Chinese herbal medicine can be found in the same herb,because of its efficacy of different active compounds.