形成丙型肝炎病毒负链复制体的相关蛋白的分析

Studies on the Proteins Related to the Replica of Hepatitis C Virus Negative-Strand RNA

【目的】确定丙型肝炎病毒 (HCV)非结构蛋白NS5B以及肝母细胞瘤株HepG2胞浆提取物中的宿主蛋白与HCV负链RNA 3′末端的特异性结合作用。【方法】用蛋白 核酸紫外交联试验分别检测NS5B以及HepG2胞浆提取物中的宿主蛋白与HCV负链RNA 3′末端的结合作用 ,用非同源RNA和非同源蛋白作为竞争物分析这种结合的特异性。【结果】NS5B以及宿主细胞内一约 4 5ku的蛋白质 (简称P4 5 )均可与HCV负链RNA 3′末端特异性结合。【结论】NS5B和P4 5是HCV负链RNA

To identify whether HCV NS5B and proteins in HepG2 cytoplasmic extracts bind specifically to the 3′ terminal sequences of HCV negative strand or not, for further study of the replica of HCV negative strand UV cross linking was used to detect the interaction of NS5B and proteins of HepG2 with the 3′ terminal sequences of HCV negative strand, the specificity of interaction was identified by using heterogeneous RNA and heterogeneous proteins as competitors in UV cross linking NS5B and a host protein with approximate molecular mass of 45 ku(P45)could specifically bind to the 3′ terminal sequences of HCV negative strand [Conclusion] NS5B and P45 are two components of the replica of HCV negative strand