一氧化氮在舌鳞癌血管生成中的作用

Role of Nitric Oxide in Angiogenesis in Squamous Cell Carcinoma of Tongue

【目的】探讨一氧化氮 (NO)在舌鳞癌血管生成中的作用。【方法】原位杂交检测 6 8例舌鳞癌活检组织常规石蜡包埋标本中诱导型一氧化氮合酶 (iNOS)mRNA的表达 ,分别以血管内皮生长因子 (VEGF)多克隆抗体、Ⅷ因子单克隆抗体免疫组化检测上述舌鳞癌标本中VEGF的表达和微血管密度 (MVD)。【结果】①舌鳞癌iNOSmRNA表达阳性组微血管密度显著高于阴性组 (P <0 0 0 1) ;在iNOSmRNA表达阳性组 ,iNOSmRNA阳性表达水平与MVD呈高度显著性正相关 ,r =0 6(P <0 0 0 1)。②舌鳞癌iNOSmRNA表达阳性组VEGF阳性率显著高于阴性组 (P <0 0 5 ) ;iNOSmRNA阳性组中 ,iNOSmRNA阳性表达水平与VEGF阳性表达水平呈显著性正相关 ,r=0 5 (P <0 0 5 )。【结论】iNOSmRNA阳性表达与舌鳞癌血管生成正相关 ,NO在舌鳞癌血管生成中起着重要作用。

To explore the role of nitric oxide(NO) in angiogenesis of squamous cell carcinoma of tongue. In situ hybridization assay was used to detect the expression of inducible nitric oxide synthase (iNOS) mRNA and streptavidin peroxidase immunohistochemistry assay was used to detect microvessel density(MVD) and the expression of vascular endothelial growth factor(VEGF) in 68 cases of biopsy specimens of tongue carcinoma tissues. ① Microvessel density count was significantly higher in the group of positive expression of iNOS mRNA of tongue carcinoma than that in the negative group(P<0 001) The count of microvessel was rising with the level of postive expression of iNOS mRNA, relative coefficient was 0 6. ② The positive expression of VEGF, in which the expression of iNOS mRNA of tongue carcinoma was positive, was significantly higher than that in the negative group(P<0 05). The level of postive expression of VEGF was also rising with the level of positive expression of iNOS mRNA, relative coefficient was 0 5.[Conclusions] A positive correlation was found between the positive expression of iNOS mRNA and angiogenesis of squamous cell carcinoma of tongue. NO plays an important role in angiogenesis of squamous cell carcinoma of tongue.