环孢素A、抗生素、硒对大鼠心肌组织中CD4^+/CD8^+淋巴细胞浸润的影响

Effects of Cyclosporine, Antibiotic and Selenium on Infiltration of CD4^+ and CD8^+ Lymphocytes in Rats′ Myocardium

将同批Wistar大鼠分为对照、环孢素A、抗生素以及微量元素硒组 (每组各 6只 )。于给药 2 4h取心脏标本 ,冰冻切片 ,常规染色和CD4 + /CD8+ 单克隆荧光抗体免疫组化标记 ,于普通光学显微镜及荧光显微镜下对心肌组织中淋巴细胞进行计数和分类。常规染色片中 ,环孢素A组、抗生素和服硒组心肌组织中浸润的淋巴细胞总数均低于对照组 (P <0 .0 1 ) ;降低程度为环孢素A组 >服硒组 >抗生素组。免疫组化片中 ,环孢素A组和抗生素组心肌组织中浸润的CD4 + 和CD8+ 淋巴细胞数低于对照组。结果提示 :环孢素A、抗生素、微量元素硒均可抑制大鼠淋巴细胞对心肌组织的浸润 ,主要通过同时抑制CD4 + 和CD8+ 淋巴细胞在心肌组织中的浸润功能而起作用。

Although cyclosporine and antibiotics have been widely used in clinical for immune suppressing in organ transplantation and antimicrobial in bacterial infection respectively, but their effects on CD4 + and CD8 + lymphocytes infiltration in myocardium have been unknown. In the present study we accounted CD4 + and CD8 + lymphocytes by immuno-histochemistry, in myocardium of rats which had separately orally administrated with cyclosporine and antibiotic and selenium for 24 h, and compared to the control group. Results showed that both of CD4 + and CD8 + lymphocytes accounts were significantly decreased (P<0.01) in three experimental groups than the control. The cell accountings towards cyclosporine were lower than antibiotics and lower than selenium. These data suggest that all of cyclosporine and antibiotics and selenium evidently suppress CD4 + and CD8 + lymphocytes infiltration in myocardium, and display a different suppressing.