抑癌基因p27对增生性瘢痕成纤维细胞增殖及DNA合成的影响

Effects of tumor suppression gene p27 on proliferation and DNA synthesis of hypertrophic scar fibroblasts

目的 探讨抑癌基因 p2 7对增生性瘢痕的作用 .方法 取体外培养的人增生性瘢痕成纤维细胞 (HTs Fb) ,将构建的 pc DNA3- p2 7真核表达质粒体外转染 HTs Fb,经 G418筛选获得稳定表达的细胞克隆 ,用 MTT和 3 H- Td R掺入法观察抑癌基因 p2 7对 HTs Fb增殖及 DNA合成的影响 .结果 抑癌基因 p2 7对 HTs Fb增殖及 DNA合成均有明显抑制作用 .结论 抑癌基因 p2 7可能通过抑制成纤维细胞增殖及DNA合成抑制瘢痕增生 .

AIM To investigate the effect of tumor suppression gene p27 on the forming of hypertrophic scars. METHODS The eukaryotic expression plasmid pcDNA3-p27 for tumor suppression gene p27 was transduced into cultured human hypertrophic scar fibroblasts in vitro. MTT and 3H-TdR incorporation were applied to measure the effect of p27 on the proliferation and DNA synthesis of hypertrophic scar fibroblasts. RESULTS Tumor suppression gene p27 inhibited the proliferation and DNA synthesis of hypertrophic scar fibroblasts. CONCLUSION Tumor suppression gene p27 may inhibit scar forming by inhibiting the proliferation and DNA synthesis of fibroblasts.