川芎嗪对缺血再灌注损伤肾脏细胞凋亡的影响

Influence of ligustrazine on cell apoptosis in rat kidneys with ischemia-reperfusion injury

目的 探讨川芎嗪对缺血再灌注损伤大鼠肾脏细胞凋亡的影响及凋亡与再灌注损伤的关系 .方法 观察川芎嗪注射液干预下 ,大鼠肾脏缺血 6 0 min,再灌注 2 4h后 ,血浆超氧化物歧化酶 (SOD)、脂质过氧化物丙二醇 (MDA)、内皮素 - 1(ET- 1)变化 ,及其对肾脏细胞凋亡的影响 .结果 川芎嗪治疗组和缺血再灌注组血浆 SOD分别是 (10 3± 18) KNU· L- 1和 (88± 14) KNU· L- 1 ,MDA分别是 (9.0± 0 .8)μmol· L- 1和 (10 .7± 0 .9) μmol· L- 1 ,ET- 1分别是 (131± 43) ng· L- 1和 (175± 47) ng· L- 1 ,川芎嗪治疗组 SOD水平显著性高于缺血再灌注组 (P<0 .0 5 ) ,而 MDA和 ET- 1水平显著性低于缺血再灌注组 (P<0 .0 5 ) .川芎嗪组和缺血再灌注组肾脏细胞凋亡指数分别是 (5 .75± 3.0 2 ) %和 (8.97± 3.41) %,川芎嗪组显著性低于缺血再灌注组 (P<0 .0 5 ) .结论 川芎嗪减轻肾脏缺血再灌注损伤 ,降低肾脏细胞凋亡指数 .

AIM To investigate the influence of Ligustrazine on cell apoptosis in rat kidneys with ischemia-reperfusion injury and the relationship between renal cell apoptosis and ischemia-reperfusion injury. METHODS The changes of plas-ma superoxide dismutase (SOD), malondialdehyde (MDA) and endothelin-1 (ET-1) as well as the influence of Ligustrazine on cell apoptosis were observed for 6 minutes 24 hours after the reperfusion in rat kidneys of ischemia. RESULTS The levels of plasma SOD, MDA and ET-1 in the Ligustrazine group were (103±18) KNU·L -1, (9.0±0.8) μmol·L -1 and (131±43) ng·L -1, respectively and those in the ischemia-reperfusion group were (88±14) KNU·L -1, (10.7±0.9) μmol·L -1 and (175±47) ng·L -1, respectively. The levels of SOD in the ligustrazine group were significantly higher than those in the ischemia reperfusion group (P<0.05), but the levels of plasma MDA and ET-1 were significantly lower than those in the ischemia-reperfusion group (P<0.05). The indexes of renal cell apoptosis in the ligustrazine group and the ischemia-reperfusion group were (5.75±3.02)% and (8.97±3.41)%, respectively and there was a significant difference between the two groups. CONCLUSION Ligustrazine reduces renal ischemia-reperfusion the injury and decreases indexes of renal cell apoptosis.