缺血后处理对缺血再灌注大鼠心肌梗死和心肌细胞凋亡的作用(英文)

Effects of ischemic postconditioning on myocardial apoptosis and infarction in rabbits with acute myocardial ischemia and reperfusion

目的 观察在体情况下缺血后处理 (IPC)对兔心肌梗死范围及缺血心肌细胞凋亡的影响 ,并与缺血预处理 (IP)心脏保护作用比较 .方法 采用兔心肌缺血 /再灌注模型 ,在缺血后、再灌注前多次短暂再灌 /停灌处理 .以 Even's blue-TTC法检测心肌梗死范围 ,TU NEL方法检测缺血心肌细胞凋亡 .结果 与对照组相比 ,缺血后处理明显减小心肌梗死范围 (12 .5± 5 .4%vs对照组 2 6 .7± 6 .7%,P<0 .0 1) ,缺血区心肌凋亡指数明显下降 (14.6± 7.4vs对照组 30 .4± 12 .3,P<0 .0 5 ) .结论 对于已缺血心肌 ,再灌前予多次短暂复灌、停灌处理具有与 IP类似缩小心梗范围作用 ,IPC对缺血心肌的保护效应可能与其抑制缺血心肌细胞凋亡有关 .

AIM To observe the effects of ischemic postconditioning (IPC) on cardiomyocyte apoptosis and myocardial infarct size in myocardial ischemia/reperfusion rabbits. METHODS Rabbit acute myocardial ischemia/reperfusion model was used and the heart was conditioned either pre-or post-myocardial ischemia followed by reperfusion. Cardiomyocyte apoptosis and myocardial infarct size were determined by in situ TDT-mediated dUTP nick end labeling (TUNEL) and Evan's blue-TTC dual staining, respectively. RESULTS Compared with those in the control group, both cardiomyocyte apoptotic index and myocardial infarct size were significantly reduced in IPC group [apoptotic index: (15±7)% vs (30±12)% of control, P<0.05; infarction:(13±5)% vs (27±7)% of control, P<0.01, respectively]. No significant difference was found between ischemic preconditioning (IP) and IPC groups. CONCLUSION Ischemic postconditioning, like ischemic preconditioning, exerts an anti-apoptotic effect, which may contribute partly to the ultimate reduction of myocardial infarction in acute myocardial ischemia-reperfusion.