博来霉素与卡氮芥体外抑制颅咽管瘤的比较

Comparison inhibitory effect of bleomycin and carmustine on craniopharyngiomas cultured in vitro

目的 :比较卡氮芥与博来霉素对颅咽管瘤细胞的体外抑瘤效果。 方法 :体外原代培养新鲜颅咽管瘤细胞 ,然后分别加入浓度为 0 .0 1、0 .1和 1.0 g/L的博来霉素或卡氮芥培养液进行培养 ,在不同时段计数以绘出抑瘤曲线 ,培养 6 d后以 ATP荧光测定法测定抑瘤效果。结果 :加药后肿瘤细胞明显减少 ;0 .0 1、0 .1和 1.0 g/L的博来霉素和卡氮芥的相对 ATP含量 (×10 - 1 4 mol/L)分别为 30 5 .5 8± 6 1.11、16 4.5 0± 5 3.0 3、1.14± 0 .45和 1.0 1± 0 .82、0 .6 8± 0 .19、0 .0 4± 0 .0 3,同一浓度组中卡氮芥抑瘤效果强于博来霉素。较低浓度组博来霉素无显著抑制颅咽管瘤作用 ,而不同浓度组卡氮芥均有显著抑瘤作用。 结论 :卡氮芥体外抑制颅咽管瘤的效果强于博来霉素 ,有应用于颅咽管瘤囊内化疗的潜在价值。

Objective: To compare the inhibitory effect of carmustine(BCNU) and bleomycin on craniopharyngiomas in vitro. Methods: Cells were successfully cultured in vitro from the fresh specimens, then the culture medium with bleomycin or BCNU at different concentration was added. The tumor inhibitory curve-line was drawn based on the cell number at different time points. After cultured for 144 h, ATP-luminescence assay was applied to test the antitumor effect. Results: The cell number decreased rapidly when the medium was added. The decreasing speed was faster in BCNU medium than that in bleomycin medium at the same concentration. The bleomycin medium showed no significant inhibitory effect except for the one at 1.00 g/L. However, regardless of the concentration, BCNU medium inhibited the cells effectively. Conclusion: BCNU has stronger inhibitory effect on craniopharyngiomas cells than bleomycin, it can be used to treat this tumor