慢性给锂盐对大鼠脑内钙调素活性的影响

THE EFFECT OF CHRONIC LITHIUM. ON CALMODULIN ACTIVITY IN RAT BRAIN

锂盐是一种防治情感疾患的有效药物,其作用机制尚未阐明.近十几年来有关锂盐作用机制的研究越来越多地集中到锂盐与第二信使系统的关系上,尤其是锂盐对腺苷酸环化酶系统的作用,有不少人做了大量的工作,但未得到一致的结论,而有关锂盐对钙调素(CaM)的作用的报道极少.本实验采用磷酸二酯酶法测定慢性给锂盐后大鼠脑内CaM含量的变化,初步探讨锂盐作用可能的分子机制.实验结果表明,慢性给锂盐后大鼠各脑区内CaM含量发生了明显变化,提示锂盐可能是通过改变脑内CaM的含量从而发挥其作用的.

Lithium(Li) salt is. a kind of effective drug commonly used in the treatment and prevention of mental disorders, but its active mechanism is still not well understood. Getting on for twenty years, many research have been reported on relationship between Li salt and second messenger system, especially the effect of Li salt on adenylate cyclase. Identical result is not obtained and there are few reports on brain calmodulin (CaM) activity change by Li salt. The aim of present expcri- ment is to determine the content of CaM in raet brain by phosphodiesterase (PED) method after chronic application of Li salt and discuss preliminary the molecular mechanism of Li salt action. The result showed that marked change has happened in the content of CaM in rat brain after chronic application of Li salt. It is ins-plied that Li salt brings its action into play probably by changing the content of CaM in rat brain