小米液态发酵生产红曲色素技术研究

Study on the Monascournarin Production by Submerged Fermentation Using Millet

研究了以小米为原料液态发酵生产红曲色素的发酵培养基组成及相关工艺参数的选择。结果表明,优化的发酵培养基组成为:小米粉11.0%、麦芽粉2.0%、谷氨酸钠0.4%及少量无机盐;摇瓶发酵工艺条件为:装液量75mL/500mL三角瓶或45mL/250mL三角瓶,接种量7%～8%,发酵液起始pH5.0,发酵温度32℃,发酵时间80h,成熟醪液红曲色价127个单位。

It described the fermentation medium and conditions of monascus pigment production by submerged fermentation using millet. The optimized medium contained: millet 11.0%,malt 2.0%,monosodium glutamate 0.4% and some inorganic salts. The fermentation parameters were obtained: pH5.0 in the beginning, filling 45 mL medium in 250mL flask or 75mL in 500mL flask, seeding 7%～8%,fermentation temperature 32℃, the time 80 hours. The color value reached above 127U in the liquid.