扇贝裙边提取物抗动脉粥样硬化作用及其机理研究

A Study of the Extract of Scallop Skirt on Anti-atherosclerotic Action and its Related Mechanisms

目的 探讨扇贝裙边提取物（ESS）抗动脉粥样硬化（AS）作用,并研究其对血管平滑肌细胞（VSMCs）增殖的影响。方法 1.通过喂饲高脂饲料建立鹌鹑AS模型,观察ESS对血脂水平、组织中脂质含量及动脉粥样硬化斑块形成的影响。2.采用20％胎牛血清（FBS）、碱性成纤维细胞生长因子（bFGF）所诱导的大鼠VSMCs增殖模型,通过细胞计数、结晶紫染色及MTT比色法研究ESS抑制VSMCs增殖作用,并运用免疫组织化学技术（LSAB法）观察ESS对增殖VSMCs的增殖细胞核抗原（PCNA）和血小板衍化生长因子（PDGF）表达的影响。结果 1.与模型组比较,ESS组动物主动脉、冠状动脉内膜AS斑块形成程度明显减轻（P<0.05、P<0.01）,血清总胆固醇（TC）、甘油三酯TC、TG含量均显著降低（P<0.05、P<0.01）。2.ESS各剂量组VSMCs数目及吸光度均明显少于模型组（P<0.05、P<0.01）,并能逆转VSMCs增殖时PCNA、PDGF的表达增强（P<0.01）。结论 ESS具有明显调血脂、抗AS作用,并能显著抑制VSMCs增殖。提示ESS的抗AS作用可能与其调血脂、抑制VSMCs增殖有关。

Objective: To study the action of anti-atherosclerosis (AS) and the effect of the extract of scallop skirt(ESS) on vascular smooth muscle cells(VSMCs) proliferation. Methods 1. In order to observe the effect of ESS on serum and tissue lipids level and atherogenic plaque, we established experimental atherosclerotic quails by feeding high lipid forage. 2. The inhibitory effects of ESS on the proliferation of rat VSMCs induced by 20% fetal bovine serum (FBS) or basic fibroblast growth factor (bFGF) were investigated in vitro culture by cell counting, crystal violet staining and MTT assays measuring. At the same time, we observed the effect of ESS on the expression of proliferating cell nuclear antigen (PCNA) and platelet-derived growth factor (PDGF) of proliferating VSMCs through immunohisto-chemi-cal technique (LSAB method). Results 1. Compared with model group, the atherosclerotic plaque of aortic and coronary arterious endothelium in ESS group was significantly decreased (P<0. 05,P<0. 01). The serum total cholesterol (TO, triglyceride (TG), low density lip-oprotein-cholesterol (LDL-C), very low density lipoprotein-cholesterol (VLDL-C), apoli-poprotein B (ApoB), and TC and TG in heart and aorta of ESS group were obvious lower than those of model group (P<0. 05.P<0. 01). 2. The number and absorbance of VSMCs in ESS groups were less than model group (P<0. 05, P<0. 01). Furthermore, ESS reversedthe enhanced expression of PCNA and PDGF (P<0. 01). Conclusion ESS possessed significant anti-atherosclerotic action, which might be related to the adjustment of serum lipid levels and the inhibition of VSMCs proliferation.