吸入丙酸倍氯米PLGA纳米缓释微囊在哮喘治疗中的作用

Experimental Research of Beclometasone Dipropionate (BDP)Nanosphere in the Treatment of Asthma

目的 :本文对比研究丙酸倍氯米松PLGA纳米缓释微囊在哮喘发病中的治疗作用。方法 :以聚乳酸 -聚羟基乙酸共聚物 (PLGA)作为微囊。健康豚鼠随机分组 ,利用卵蛋白致敏诱发哮喘模型。随后测定各组血及肺泡灌洗液中炎性细胞的数目及分类 ,并取肺组织做病理检查。结果 :(1)丙酸倍氯米松PLGA纳米缓释微囊为表面光滑的球形微囊 ,粒径平均质 12 6 .4mm ,呈正态分布。 (2 )治疗后纳米控释与普通药物治疗组均取得良好的治疗效果 ,与对照组相比血及肺泡灌洗液中白细胞总数及分类进行F检验 ,组间有显著差异 (P <0 .0 5 ) ,纳米控释组表现出良好的药物控释性。 (3)病理显示试验组气道炎症明显减轻。结论 :PLGA纳米粒子可以作为抗哮喘药物丙酸倍氯米松的有效载体 ,更适合吸入的药物剂型。

Objective: To prepare BDP nano sustained-release sphere and survey its diammond distribution, also to study its therapeutic action asthma. Methods: With PLGA as matrix materials, we adopted ultrasound emulsion/liquor volatilization method to prepare PLGABDP nano pareicles (NP), proceeding the appearance singns of PLGA-BDP-NP,making use of scanning elextrcity microsopy A and laser scattering experiment to look into the morphorlogy of nanoparticles and survey its diameter distribution; by means of HPLC we carry out the drug-carrying coefficient (ratio) of the nanoparticles. Then 40 healthy guinea pigs were randomly divded into four groups: the normal control group(A), the model control group(B), the BDP theerapy group (C), the BDP nano capsule therapy group (D). These guinea pigs are sensitived and induced asthmatie attack by OVA. We determine the inflammative cells in their blood and BALF, and take pathological examination. Results: 1. Observation of EM showed that PLGA-BDP-NP nanospheres have smooth surfaces; 2. The average diameter value is 126.4nm, under normal distribution. 3. Results of asthmatic therapy: The total number of WBC?eosophinole?granulocyte in blood &BALF is seperstively compared among four groups, difference is significant( P <0.05). Both C group and group have ideal effects on the asthma, between them differece is not singnificant( P <0.05), BDP nano group(D) perform remarked drug control-releasing character. 4. Pathological results in the section of the model group, the constructure of bronchial and lung is derangement, smooth muscle obviously hyperplasia, the wall of alveoli is congestive and edematous; while in the BDP therapy group and BDP nano capsule therapy group, infilmmation is obviously improvement. Conclusion: Nanolarticles may serve as availability carrier of BDP, and can be succeessesfully used in the prepartion of its inhaled dose, suitable fot inhalation of drugs so as to play more efficiency to treat asthma.