产超广谱β内酰胺酶产酸克雷伯菌的医院感染流行分析

An outbreak of extended-spectrum beta-lactamase-producing Klebsiella oxytoca in a surgical ward

目的 研究同一时期、同一病房、不同病人临床分离的 8株多重耐药产酸克雷伯菌的同源特性。方法 对 8株产酸克雷伯菌进行药物敏感性试验、超广谱 β内酰胺酶 (ESBLs)表型测定、等电聚焦电泳、脉冲场凝胶电泳、聚合酶链反应 (PCR)及PCR产物测序等分子生物学分析。结果 8株产酸克雷伯菌为产CTX M 3型ESBLs的亲缘关系密切的同源菌。结论 8株产酸克雷伯菌在同一病房引起过小规模暴发流行 ,系同源菌。

Objective To study 8 isolates of multiple antibiotic-resistant Klebsiella oxytoca in 8 different patients from one surgical ward. Methods Susceptibility test, extended-spectrum β-lactamases (ESBLs) screening methods and some molecular biological methods were performed. Results 8 isolates of Klebsiella oxytoca were verified as closely related homologous CTX-M-3-producing strains. Conclusions The little outbreak was caused by 8 closely related homologous strains in one ward.