己酮可可碱对日本血吸虫病肝纤维化小鼠肝脏TGF- β1和 、 型胶原表达的影响

Effect of Pentoxifylline on the Expression of Hepatic TGF-β1, Type I and Type III Collagen in Mice with Liver Fibrosis due to Schistosoma japonicum Infection

目的 研究己酮可可碱 (pentoxifylline,PTX)对日本血吸虫病肝纤维化小鼠肝脏转化生长因子β1(TGF-β1)和 、 型胶原含量的影响。 方法 4 0只血吸虫病肝纤维化小鼠均分 4组 ,对照组不作任何治疗。吡喹酮组 5 0 0mg/ (kg· d)治疗 2 d,高剂量 PTX组 36 0 mg/ (kg· d)治疗 8wk,低剂量 PTX组 180 mg/ (kg· d)治疗 8wk。应用免疫组化染色方法和多媒体彩色病理图文分析系统 ,观察不同剂量 PTX治疗前后肝脏 TGF-β1和 、 型胶原含量的变化。 结果 PTX对 TGF- β1和 、 型胶原含量的影响与治疗剂量有关 ,高剂量 PTX组 TGF- β1和 、 型胶原含量明显低于对照组 (P<0 .0 1) ,而低剂量 PTX组与对照组之间差异无统计学意义 (P>0 .0 5 )。 结论 高剂量 PTX治疗血吸虫病肝纤维化小鼠可显著降低其肝脏 TGF-β1和 、 型胶原的含量。

Objective To study the effect of pentoxifylline (PTX) on the content of hepatic TGF-β1, type Ⅰ and type Ⅲ collagen in schistosome-infected mice with liver fibrosis. . Methods. Forty mice with schistosomiasis were divided into four groups: one group as control without any treatment, other three were treated with praziquantel 500 mg/(kg·d) for 2 d, high dose PTX 360 mg/(kg·d) for 8 wk, and low dose PTX 180 mg/(kg·d) for 8 wk respectively. Immunohistochemical technique and multimedia color pathographic analysis system were applied to observe the content of hepatic TGF-β1, type Ⅰ and type Ⅲ collagen in mice infected with S.japonicum before and after treatment. . Results . The effect of PTX on the content of hepatic TGF-β1, type Ⅰ and type Ⅲ collagen in mice was related to the dosage of PTX. High dose PTX treatment significantly reduced the content of TGF-β1, type Ⅰ and type Ⅲ collagen compared to the control (P<0.^01), whereas no difference was found between the group of low dose PTX treatment and control (P>0.^05). . Conclusion . High dose PTX treatment could reduce the content of hepatic TGF-β1, type Ⅰ and type Ⅲ collagen significantly in schistosome-infected mice with liver fibrosis.