AGT基因5’侧翼C532T突变和M235T多态性与X射线工作者原发性高血压的关系

Association analysis of angiotensinogen gene 5'-flanking region C532T mutation and M235T polymorphism in essential hypertension of Chinese medical diagnostic X-ray workers

目的 探讨血管紧张素原 (AGT)基因 5’侧翼非编码区C5 32T突变同编码区M2 35T多态性之间在X射线工作者原发性高血压发病中的作用。方法 应用聚合酶链反应限制性内切酶片断长度多态性 (PCR RFLP)的方法 ,分析了 137例X射线工作者原发性高血压患者及 114例对照AGT基因 - 5 32位及M2 35T两个不同位点基因多态性。通过家族史分析了家族遗传易感性同两基因型之间的关系。结果 有家族史的高血压组AGT基因M2 35T多态性MM、MT、TT 3种基因型频率为0 0 5、0 18、0 77,无家族史的对照组基因型频率分别为 :0 0 8、0 35、0 5 7(P =0 0 31) ,T等位基因频率病例和对照分别为 0 86、0 74 (P =0 0 0 9) ;G5 32TCC、CT、TT 3种基因型频率及等位基因频率两组间差异均无显著性 ;两位点联合分析表明 :具有 5 32位T等位基因的个体 ,M2 35T多态性同高血压病密切相关 ;有无职业紧张、辐射剂量大小不同组 ,基因型分布在病例及对照组中不同。结论 5’端C5 32T突变及编码区M2 35T多态性可能共同参与了X射线工作者高血压的发病过程 。

Objective\ To investigate whether angiotensinogen(AGT)gene 5'\|flanking region C532T mutation and M235T polymorphism are associated with essential hypertension(EH) and the joint effect of the two sites. Methods\ A case\|control study was carried out using 137 cases of essential hypertension and 114 cases of control.The C532T mutation and M235T polymorphism of the angiotensinogen gene were determined by polymerase chain reaction and restriction fragment length polymorphism (PCR/RFLP) analysis. Results\ The differences of genotype distribution of the AGT gene between cases having a family history of hypertension and controls without that history were statistically significant.(MT 0\^18,TT 0\^77 and MM 0\^05 for case genotype vs MT 0\^35,TT 0\^57 and MM 0\^08 for control, P =0 031) A correlation between M235T and C532T molecular variant of AGT gene to hypertension was found. Conclusion\ Combinations of variations in angiotensinogen gene and occupational factors might associate with essential hypertension.