摘要
目的 研究辐射远后效应对红细胞膜阴离子转运蛋白 (band 3)基因表达的影响。方法 分离 10年期骨髓型急性放射病 (ARS)患者骨髓有核细胞 ,二步法液体培养红系 ,取新生红细胞涂片 ,做band 3mRNA原位杂交。抽提外周血有核细胞总RNA ,进行RT PCR ,以 β actin作内参照 ,半定量分析band 3mRNA表达丰度。结果 ARS新生红细胞中band 3mRNA原位杂交显色不均、淡染 ,定量分析显示 1 2患者band 3mRNA表达强度减弱。结论 辐射远后效应可能在转录水平影响红细胞膜阴离子转运蛋白基因表达 ,使转录产物减少 ,翻译产物功能下降即阴离子转运能力减弱 。
Objective\ To study the delayed effect of radiation on the gene expression of anion exchanger (band 3 protein) in erythrocyte membrane. Methods\ Two\|step liquid culture of erythroid cell was conducted by isolating blasts freshly from patients with bone marrow form of acute radiation sickness (ARS) at the 10 th year after exposure to radiation and the band 3 gene expression in the culture cells was observed using B3 mRNA in situ hybridization.The expression level of band 3 gene in total RNA from peripheral blood mononuclear cells of ARS patients was analyzed by semi\|quantitative reverse transcriptase\|polymerase chain reaction (RT\|PCR).β\|actin was used as the internal reference. Results\ Weak and uneven location of band 3 mRNA positive stain in the cultured late erythroblasts was shown by using in situ hybridization.The expression level of band 3 mRNA in half of the ARS patients was decreased. Conclusion\ The results suggest that the delayed effect of radiation might be exerted on band 3 mRNA transcription,resulting in lowered level of the transcription product and its weakened function,e.g.the decreased anion transport of anion exchanger,and prolonged course of erythropoiesis.
出处
《中华放射医学与防护杂志》
CAS
CSCD
北大核心
2002年第4期275-276,共2页
Chinese Journal of Radiological Medicine and Protection
基金
总后卫生部专项基金拔款资助项目 (98M0 81)