大豆磷脂脂质载体与胆盐表面活性剂的体外相互作用

Interaction of soya phospholipid vesicle and bile salt surfactants in vitro

目的 研究大豆磷脂脂质体与胆盐表面活性剂的体外相互作用。方法 采用旋转薄膜蒸发法结合挤压过滤工艺制备大豆磷脂脂质体 ,经透射电镜观察脂质体的外观形态 ,通过胆盐和脱氧胆盐研究其与脂质体之间的相互作用 ,将 5 0 0nm波长处的可见光吸收值评价胆盐 脂质体混悬液的浊度 ,并测定脂质体的粒径变化情况。结果 所制得脂质体呈明显的双层膜圆球型结构 ,其平均粒径为 2 17nm ,跨距为 0 .838。在胆盐加入的初期 ,由于胆盐和脂质体形成混合胶团 ,致使脂质体的粒径和浊度值稍有增加 ,进一步加入的胆盐使脂质体的粒径和浊度值下降。结论 胆盐和脂质体间的体外相互作用与混合胶团结构变化密切相关 ,可反映脂质体的载药和释药特性 ,对脂质体的体内过程研究具有重要意义。

OBJECTIVE: To investigate the interaction of soya phospholipid liposomes and the bile salts surfactant in vitro. METHODS: The liposomes were prepared by extrusion method after reverse phase evaporation technique. Transmission electron microscopy was used to detect the appearances of the liposomes. Sodium cholate and sodium deoxycholate were used to study their interaction with the liposomes. The turbidities for the mixture of sodium cholate and sodium deoxycholate were evaluated by the visible spectrometry at the wavelength of 500 nm. And the changes of the diameters of liposomes were also tested to examine the effect of bile salts on liposomes. RESULTS: The prepared liposomes were sphere in shape with the mean diameter of 217 nm and span of 0. 838. The phospholipid bilayer structure was seen clearly under transmission electron microscopy. The diameters and turbidities of liposomes were increased a little as the result of mixed micelles formation during the different stages for the structure changes of surfactant-liposomes micelles. The further added bile salts decreased the diameters and turbidities of liposomes. CONCLUSION: The interaction of bile salts and liposomes, followed by the structure changes of the mixed lipid micelles, was important for the further study of the behaviors of liposomes in vivo. The drug loaded and release properties of liposomes may also be reflected upon the interaction process.