血流切应力对动脉内皮细胞凋亡影响的在体研究

Influence of Flow Shear Stress on Apoptosis of Arterial Endothelium in vivo

探讨动脉血流受阻后壁切应力 (WSS)变化对动脉内皮细胞 (AEC)凋亡的影响。用 6 0只实验兔建立动脉血流减少模型 ,在术后 0～ 30 d 8个不同时点 ,AEC铺片 Tunel原位末端标记法 ,计量 AEC的凋亡率。结果发现 :在 WSS降低后 1～ 7d,AEC凋亡率显著增高 ,3d达到峰值。随着 WSS的递增 ,术后 14、30 d AEC的凋亡率又恢复至正常对照水平。提示 WSS的变化可显著影响 AEC的凋亡状况 ,其中

To explore the changes of wall shear stress(WSS) effect on arterial endothelial cell(EC)apoptosis after reducing arterial blood flow. The reducing flow model was established in 60 rabbits. Endothelial stretched preparations were made at 8 different time intervals from 0 to 30 days . The apoptosis rate of arterial endothelial cells(AEC) was measured with TdT-mediated dUTP-biotin nick end labeling(TUNEL)method. The results showed that the apoptosis rate of AEC was significantly higher from 1 day to 7 days after decreasing WSS than that of control , which peaked on day 3. While with progressively increasing in WSS, the apoptosis rate restored to the level of control from 14 days to 30 days. These suggest that the apoptosis state of AEC might be markedly influenced by the changes of WSS. The persist decreasing of WSS may be the important factor which induces the cell apoptosis.