脐血超氧化物歧化酶活性的变化及其临床意义

The Clinic Significance and Changes Of Superoxidation Dismutase Activity in Umbilical Blood

目的研究围生期窒息、妊娠高血压综合症、妊娠晚期羊水过少、胎盘老化等妊娠并发症者脐血超氧化物歧化酶 (SOD)活性的变化及其临床意义。方法采用邻苯三酚自氧化法 ,分别检测 30例围生期窒息、30例妊娠高血压综合症、30例妊娠晚期羊水过少、30例胎盘老化和 30例正常妊娠足月顺产的胎儿脐血SOD的活性。结果不同妊娠分娩状态胎儿脐血SOD活性的差异有显著性意义 (F =2 6 .85 32 ,P <0 .0 0 1 )。围生期窒息组和妊娠晚期羊水过少组脐血SOD活性高于对照组 ( 4 0 .1 4± 1 2 .6 1u/ml,39.4 0± 9.80u/ml和 2 9.39± 7.70u/ml,q’ =6 .4 0 6 8,P <0 .0 0 1 ,和 q’ =5 .96 5 8,P <0 .0 0 1 )。而妊娠高血压综合症和胎盘老化组脐血SOD活性低于对照组 ( 2 1 .89± 4 .6 9u/ml,2 30 6± 5 .5 4u/ml,q’ =4 .4 6 99,P>0 .0 0 1 ,和 q’ =3.772 6 ,P >0 .0 1 )。

Objective To research the differences of superoxide dismutase (SOD) activity in umbilical blood from newborns with perinatal asphyxia and other pregnancy complications such as pregnant hypertension syndrome,oligoamnios in late pregnancy and aging of placenta etc,in comparison with that from normal pregnancy-bearing.Methods 150 pregnant women aged 21-29 years were divided into five groups:the perinatal asphyxia group (n=30),the pregnant hypertension group (n=30),the oligoamnios group (n=30),and the aging of placenta group (n=30),and the control group (n=30).The activity of SOD in umbilical blood was measured by autoxidation of pyrogallol.Results The SOD activities in umbilical blood were significant differences in the groups (F=26.8532, P&lt;0.001).The SOD activities of the oligoamnios and prenatal asphyxia groups were significantly higher than that of the control group (40.14±12.61 u/ml,39.40±9.80 u/ml and 29.39±7.70 u/ml,q’=6.4068,P&lt;0.001 and q’=5.9658,P&lt;0.001),and SOD activities in pregnant hypertension group and oligoamnios groups were significantly lower than that of the control group (21.89±4.69 u/ml,23.06±5.54 u/ml,q’=4.4699,P&lt;0.001 and q’=3.7726,P&lt;0.01).Conclusion The activity of SOD in umbilical blood can act as a biochemical index for diagnosing the perinatal asphyxia and oligoamnios,and assessing the placental dysfunction complicated by pregnant hypertension and aging of placenta.