子宫肌瘤组织中孕激素受体和表皮生长因子受体及细胞增殖相关抗原的表达

Expression of Progesterone Receptor,Epidermal Growth Factor Receptor and Cell Proliferation Associated Antigen in Human Uterine Leiomyoma

目的 :探讨孕激素 (P)和表皮生长因子 (EGF)对子宫肌瘤生长的影响。方法 :采用免疫组化SP法和计算机图象分析系统 ,定量分析子宫肌瘤患者的子宫肌瘤组织及子宫肌组织内孕激素受体 (PR)、表皮生长因子受体(EGFR)和细胞增殖相关抗原 (Ki- 6 7)的含量。结果 :(1)PR、EGFR和Ki- 6 7在子宫肌瘤组织中的表达明显高于子宫肌组织。 (2 )PR、EGFR在子宫肌瘤组织中的表达分泌期显著高于增生期 ,但在子宫肌组织中无变化 ;Ki- 6 7在两种组织中的表达分泌期明显高于增生期。 (3)子宫肌瘤组织中PR、EGFR与Ki - 6 7呈正相关。结论 :P和EGF在子宫肌瘤生长中起重要作用 。

Objective To study the role of progesterone(P) and epidermal growth factor(EGF) in the growth of leiomyoma. Methods The tissues of leiomyoma were obtained from 30 uterine leiomyoma women undergoing hysterectomy, with the myometrium as control. PR, EGFR and Ki-67 contents of both normal uterine myometrium and leiomyoma were quantitalively analysed by SP method and image analyser system(IAS). Results (1) Contents of PR,EGFR and Ki-67 were significantly higher in leiomyoma than those in normal myometrium( P <0.001, P <0.0, P <0.05). (2) PR and Ki-67 contents of leiomyoma were signficantly higher than those of normal myometrium both in proliferative phase and secretory phase( P <0.05); EGFR contents were higher than those of nomal myometrium only in secretory phase( P <0.001), and showed no difference in proliferative phase( P >0.05). (3) PR and EGFR contents of leiomyoma were significantly higher in secretory phase than those in proliferative phase( P <0.01, P <0.001), while there were no difference in myometrium( P >0.05); Ki-67 contents of the two tissues in secretory phase were greater than those in proliferative phase( P <0.001). (4) PR and EGFR contents of leiomyoma were postively correlated with those of Ki-67(P<0.001). Conclusions High PR, EGFR and Ki-67 contents in leiomyoma , may be the result of local high P, EGF concentrations, which indicates that P and EGF have important effects in the growth of leiomyoma , and EGF may act as a mediator of progesterone.